rabbit polyclonal anti-mouse muc2  (Santa Cruz Biotechnology)

Journal: Cell reports

Article Title: Dynamic genetic adaptation of Bacteroides thetaiotaomicron during murine gut colonization

doi: 10.1016/j.celrep.2023.113009

Figure Lengend Snippet: (A–E) Representative cross-sections of distal mouse colon fixed in Carnoy’s solution at (A) day 1 (D1), (B) D2, (C) D4, (D) D7, and (E) D42 of colonization by Bt . Blue, DAPI staining in the epithelium; red, DAPI staining in the lumen of gut, including bacteria, debris, and shed host nuclei; green, antibody staining for MUC2. Scale bars, 10 μm. (F) Mean Bt epithelial proximity. n = 3–4/group, t test, *p < 0.05.

Article Snippet: Rabbit polyclonal anti-mouse Muc2 , Santa Cruz Biotechnology , Cat#sc-15334.

Techniques: Staining, Bacteria

Journal: Cell reports

Article Title: Dynamic genetic adaptation of Bacteroides thetaiotaomicron during murine gut colonization

doi: 10.1016/j.celrep.2023.113009

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-mouse Muc2 , Santa Cruz Biotechnology , Cat#sc-15334.

Techniques: Virus, Mutagenesis, Isolation, Recombinant, Saline, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, cDNA Synthesis, Shotgun Sequencing, Sequencing, Software, Spectrophotometry, Mass Spectrometry, Microscopy

Journal: Cell reports

Article Title: Dynamic genetic adaptation of Bacteroides thetaiotaomicron during murine gut colonization

doi: 10.1016/j.celrep.2023.113009

Figure Lengend Snippet: (A–E) Representative cross-sections of distal mouse colon fixed in Carnoy’s solution at (A) day 1 (D1), (B) D2, (C) D4, (D) D7, and (E) D42 of colonization by Bt . Blue, DAPI staining in the epithelium; red, DAPI staining in the lumen of gut, including bacteria, debris, and shed host nuclei; green, antibody staining for MUC2. Scale bars, 10 μm. (F) Mean Bt epithelial proximity. n = 3–4/group, t test, *p < 0.05.

Article Snippet: After deparaffinization and rehydration, slides were incubated in lysozyme solution at 37°C for 20 min, and then in antigen retrieval solution (10 mM sodium citrate [pH 6.0]) at 90°C for 10 min. For mucus visualization, a polyclonal rabbit anti-mouse Muc2-specific antibody (Santa Cruz Biotechnology) was diluted 1:100 in blocking buffer (Dako), applied to the slide, and incubated for 2 h in the dark at room temperature.

Techniques: Staining, Bacteria

Journal: Cell reports

Article Title: Dynamic genetic adaptation of Bacteroides thetaiotaomicron during murine gut colonization

doi: 10.1016/j.celrep.2023.113009

Figure Lengend Snippet:

Article Snippet: After deparaffinization and rehydration, slides were incubated in lysozyme solution at 37°C for 20 min, and then in antigen retrieval solution (10 mM sodium citrate [pH 6.0]) at 90°C for 10 min. For mucus visualization, a polyclonal rabbit anti-mouse Muc2-specific antibody (Santa Cruz Biotechnology) was diluted 1:100 in blocking buffer (Dako), applied to the slide, and incubated for 2 h in the dark at room temperature.

Techniques: Virus, Mutagenesis, Isolation, Recombinant, Saline, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, cDNA Synthesis, Shotgun Sequencing, Sequencing, Software, Spectrophotometry, Mass Spectrometry, Microscopy

Journal: The Journal of Biological Chemistry

Article Title: Autophagy Protects against Colitis by the Maintenance of Normal Gut Microflora and Secretion of Mucus *

doi: 10.1074/jbc.M114.632257

Figure Lengend Snippet: Primers for quantitative RT-PCR

Article Snippet: After blocking with PBS containing 3% BSA, 0.5 μg/ml rabbit anti-mouse MUC2 polyclonal antibody (H-300; Santa Cruz Biotechnology) or IgG from rabbit serum (Sigma) was added to the wells and incubated overnight.

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: Autophagy Protects against Colitis by the Maintenance of Normal Gut Microflora and Secretion of Mucus *

doi: 10.1074/jbc.M114.632257

Figure Lengend Snippet: Secretion of colonic mucins in WT and Atg7 cKO mice. Hematoxylin and eosin (A), Alcian blue-PAS (B), and anti-MUC2 (C) staining of the mouse distal colon. D, percentage of mucus-secreting crypts. E, quantification of inner mucus layer thickness. F, detection of fecal MUC2 by ELISA. G, quantitative RT-PCR. The relative quantity of Muc2 mRNA compared with the quantity of β-actin was determined by the ΔΔCt method. Error bars represent the S.D. of samples within a group. Asterisk in A–C, mucus-secreting crypt. Arrows in A–C, mucus-secreting goblet cells. Dashed line in C, mucosal surface. An unpaired Student's t test was used to compare WT versus cKO mice. **, p < 0.01; *, p < 0.05. n = 3 per group.

Article Snippet: After blocking with PBS containing 3% BSA, 0.5 μg/ml rabbit anti-mouse MUC2 polyclonal antibody (H-300; Santa Cruz Biotechnology) or IgG from rabbit serum (Sigma) was added to the wells and incubated overnight.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR